Tris(hydroxymethyl)aminomethane, research grade, Serva

Opis towaru

(TRIS, THAM, Tromethamine, Amino-2-(hydroxymethyl)-1,3-propanediol)

C4H11NO3

Mr 121.1

CAS [77-86-1]

Tris(hydroxymethyl)aminomethane (Tris) buffer quality for all standard applications, suitable for preparative purposes and for chromatography.

With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5. It is used as a component in buffer solutions in numerous applications:

• Assays in molecular biology and cytology
• Extraction of proteins or nucleic acids from cells
• In situ hybridisation procedures
• Sample and running buffers for SDS-PAGE
• TAE- and TBE buffers for agarose gel electrophoresis
• Transfer buffer for Western Blotting
• Washing buffers for immunoassays

Assay (titr.) - min. 99.0 %

A 1 cm/40 % in water 290 nm - max. 0.2

pH (5 % in water) - 10.0 - 11.5

Heavy metals (Pb) - max. 10 ppm