Tris(hydroxymethyl)aminomethane, molecular biology grade, Serva

Opis towaru

(TRIS)

C4H11NO3

Mr 121.1

CAS [77-86-1]

Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality for sensitive assays in molecular biology with very low UV absorption, DNase, RNase, Protease not detected.

With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.

It is used as a component in buffer solutions in numerous applications:

• Assays in molecular biology and cytology
• Extraction of proteins or nucleic acids from cells
• In situ hybridisation procedures
• Sample and running buffers for SDS-PAGE
• TAE- and TBE buffers for agarose gel electrophoresis
• Transfer buffer for Western Blotting
• Washing buffers for immunoassays

Assay (titr.) - min. 99.9 %

Heavy metals (Pb) - max. 10 ppm

Iron - max. 10 ppm

Arsenic (As) - max. 1 ppm

Magnesium (Mg) - max. 1 ppm