Ribonuclease A from bovine pancreas min. 80 Kunitz units/mg, lyophil., SERVA

Opis towaru

Chromatographically homogeneous preparation of Ribonuclease A (RNase A), free of DNase, protease, and salt. A major application for RNase A is the removal of RNA from preparations of plasmid DNA. It is also used in RNA sequence analysis, protection assays and removal of RNA contained in protein samples. RNase A is an endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts. The enzyme is inhibited by heavy metal ions and competitively by DNA.

Pancreatic RNase, ribonuklease, ribonucleate 3-pyrimidino-oligonucleotidohydrolase
EC 3.1.27.5 

M_r ca. 13 700

CAS No. 9001-99-4

Unit definition: 1 unit is that amount of activity which is capable of causing within 1 minute a decrease in absorbance at 300 nm equivalent to the maximum possible change in a 0.05 % solution of yeast RNA at 25 °C, pH 5.0.