Protein Test Mixture 5 for SDS PAGE, Dalton-marker proteins, low molecular weight, for SDS PAGE, Serva
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Molecular weight markers for SDS gel electrophoresis.
Reconstitute with SDS sample buffer (125 mM Tris-HCl pH 6.8, 2 % SDS, 15 % glycerol, 10 mM DTT, 0.025 % bromophenol blue, 0.025 % Orange G) to final concentration of 1 mg/ml and apply 5 µl per lane when staining with SERVA Blue G, SERVA Blue R or Coomassie®.
For silver staining, e.g. using SERVA's Silver Staining Kit (cat. no. 39076), dilute 1:5 in 1x Laemmli buffer and apply 5 µl.
Carbonic anhydrase - Mr 29 000
Trypsin inhibitor (soybean) - Mr 21 000
Cytochrome C - Mr 12 300
Trypsin inhibitor (bovine lung) - Mr 6 500
Coomassie = registered trademark of ICI Ltd.
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