PNGase F, recombinant, SERVA

Opis towaru

Recombinant glycosidase from Flavobacterium meningosepticum with faster kinetics and greater activity against native glycoproteins.

The proprietary changes made to PNGase F have been shown to have unique characteristics when compared to other commercially-available sources:

•  Does not need a denaturing step

•  Works on native glycoproteins and serum glycoproteins in only minutes at room temperature

•  Leads to a more complete glycan release compared to other commercially-available enzymes

•  Especially designed and tested for mass spectrometry imaging of tissue samples

•  Contains a His-tag for easy removal by affinity chromatography

The enzyme catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins.
Concentration: 1000 u/µl (2.0 mg/ml), supplied in 1x PBS

M_r ca. 36 000

Storage Temperature: -15 °C to -25 °C