N-Tris(hydroxymethyl)methylglycine, analytical grade, SERVA

Opis towaru

Tris(hydroxymethyl)methylglycine (Tricine) is a zwitterionic Good's buffer used in biochemistry and molecular biology. The buffer is part of the Tris buffer family and has a useful pH range of 7.4 – 8.8, pKa 20 = 8.15.
Tricine is optimal to separate peptides that are in the molecular weight range of 1 to 100 kDa in SDS-PAGE. It is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography. It is the best buffer for ATP assays involving firefly luciferase and is a scavenger of hydroxyl radicals. Tricine forms a complex with Cu(II) and is therefore used in the Lowry protein assay. The buffer is a non-toxic substitute for barbital.

Tricine
C₆H₁₃NO₅ 

M_r 179.17

CAS No. 5704-04-1 

Assay (titr.): min. 99.0 %
A 1 cm/0.1 M in water
260 nm: max. 0.04
280 nm: max. 0.02

pH 10 % in water: 4.6 - 5.2
Heavy metals (Pb): max. 5 ppm
Iron (Fe): max. 5 ppm

Storage Temperature: +15 °C to +30 °C