N-(2-Hydroxyethyl)piperazine-N'-2-ethane sulfonic acid, analytical grade, for cell culture, SERVA

Opis towaru

Tested for use in tissue culture.
At biological pH, HEPES is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 20 = 7.55). Thus, this Good's buffer is used for a wide range of applications in biological research:

•  In tissue culture to buffer cell culture media in air

•  As a component in cell culture media like DMEM, RPMI, Hanks’ solution

•  In biological solutions for the adjustment and maintenance of pH

•  For homogenization of tissue and in the preparation of cytosolic and nuclear cell extracts

•  As a buffer component in gel filtration and affinity chromatography

HEPES
C₈H₁₈N₂O₄S

M_r 238.3

CAS No. 7365-45-9

Assay (titr.): min. 99.0 %
A 1 cm/10 % in water
260 nm: max. 0.1
280 nm: max. 0.08
Heavy metals (Pb): max. 10 ppm
pH 10 % in water: 5.0 - 6.5

Storage Temperature: +15 °C to +30 °C