Endo S, recombinant, lyophilized, SERVA

Opis towaru

Endo S, a recombinant endo-β-N-acetylglucosaminidase S from Streptococcus pyogenes, plays a central role in glycoengineering strategies for the development of IgG antibodies with improved therapeutic efficacy. This enzyme will leave any human IgG with a single N-acetylglucosamine, with or without an attached fucose molecule.

•  Especially designed and tested for mass spectrometry imaging and HPLC/UPLC

•  Outstanding performance — produces in HPLC clean peaks with very high yields

•  Contains a His-tag for easy removal by affinity chromatography

The endoglycosidase Endo S has a unique accuracy for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG molecules. The enzyme hydrolyzes the β(1-4) linkage between the two core GlcNAcs of asparagine linked biantennary complex-type glycans of human IgG Fc regions.
Because the enzyme is lyophilized, there is no need for refrigerated transport and storage is at room temperature.
Concentration after reconstitution with 10 µl/50 µl/100 µl H₂O dest.: 200 u/µl (1.0 mg/ml) in 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA. Delivered with 10x reaction buffer.

Endo-β-N-acetylglucosaminidase S, Endoglycosidase
M_r 108.000

Storage Temperature: +15 °C to +30 °C