Elastase from porcine pancreas min. 200 U/mg protein, lyophil., SERVA

Opis towaru

For the degradation of proteins and peptides.

Serine proteinase with broad substrate specificity. It preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. This specificity explains its unique ability to digest native elastin, which is found in highest concentrations in the elastic fibers of connective tissues. Therefore elastase in combination with other enzymes like trypsin is frequently used to dissociate tissues which contain extensive intercellular fiber networks.

Elastase is also able to digest other proteins such as fibrin, hemoglobin, and casein, but not native collagen and keratin. In the presence of Tris, sodium sulfate or SDS enzyme activity is greatly stimulated.

One unit will hydrolyze one micromole of methoxy succinyl-alanine-alanine-proline-valine-p-nitroanilide per minute at 37 °C and pH 7.5.

DANGER

Hazard Statements H315 - H319 - H334 - H335

Precautions P280 - P284

Reaction P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311

CAS No. 39445-21-1

(Pancreatopeptidase E)

EC 3.4.21.36

M_r ca. 25 900

Storage Temperature: -15 °C to -25 °C