Dithiothreitol, molecular biology grade, SERVA

Opis towaru

Dithiothreitol, as well called DTT or Cleland's reagent, is a widely used reagent for maintaining thiol (SH) groups in reduced state. DTT quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE).

It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed.

DNase/RNase not detected. Oxidized DTT max. 0.5 %. Hygroscopic.

DTT, Cleland's reagent, Dimercapto-2,3-butanediol
C₄H₁₀O₂S₂ 

M_r 154.25

CAS No. 3483-12-3

Assay (titr.) min. 99.0 %

MP 40 - 45 °C

Storage Temperature +2 °C TO +8 °C