Dithiothreitol, analytical grade, SERVA

Opis towaru

Dithiothreitol, as well called DTT or Cleland's reagent, quantitatively reduces disulfide groups (1) and is effective in sample buffers for reducing protein disulfide bonds prior to SDS gel electrophoresis (SDS-PAGE). The reducing thiol agent cleaves disulfide bonds between cysteine residues, thus totally unfolding proteins to allow a separation according to the molecular weight. It may also be used in lysis buffer in protein sample preparations. It is not only less pungent and less toxic than 2-mercaptoethanol, but as well a seven fold lower concentration of DTT (100 mM) than of 2-mercaptoethanol (5 % v/v, 700 mM) is needed. Oxidized form of DTT max. 0.5 %. Hygroscopic.

DTT, Cleland's reagent, Dimercapto-2,3-butanediol
C₄H₁₀O₂S₂ 

M_r 154.25

CAS No. 3483-12-3

Assay (titr.) min. 99.0 %

MP 40 - 45 °C

Storage Temperature +2 °C TO +8 °C