Deoxyribonuclease I from Bovine Pancreas min. 3000 Kunitz units/mg, lyophil., SERVA

Opis towaru

Deoxyribonuclease I, or DNase I

• is used to nick DNA as a first step to incorporate labelled bases into DNA
• is suitable for DNase footprinting
• improves yield in primary cell isolation by removing extracellular DNA
• reduces viscosity in protein samples by removal of DNA contamination

DNase I is an endonuclease that cleaves phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. It preferentially cleaves double-stranded DNA and, in the presence of Mg2+, single-stranded DNA.

CAS No. 9003-98-9

(DNase I, Deoxyribonucleodepolymerase, Deoxyribonucleate 5'-oligo-nucleotide hydrolase, Desoxyribonuclease, Nuklease, Ribonuklease, )

EC 3.1.21.1

M_r 31 000

Unit definition: 1 Kunitz unit catalyses an increase in absorbance of 0.001 at 260 nm per minute at 25 °C, pH 5.0, when exposed to highly polymerised calf thymus DNA (1).

Activity: min. 3000 Kunitz units/mg lyophilisate