Coomassie(R) Brilliant Blue G 250, SERVA

Opis towaru

Coomassie® Brilliant Blue G 250 is a triphenylmethane dye used in protein gel electrophoresis for detection of proteins and with the Bradford method to determine protein concentration. G 250 is differentiated from the R 250 Coomassie stain by the addition of two methyl groups and the slightly greenish tint to its blue color. The dye may exist as a cation (red form) at a pH below 0 with an absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an absorbance peak around 650 nm. The blue, anionic form is what binds with amino acid residues, such as arginine or aromatics, to form a stable complex. Corresponds to SERVA Blue G (cat. no. 35050).

C.I. 42655 

C₄₇H₄₈N₃O₇S₂·Na

M_r 854.0

CAS No. 6104-58-1

λmax. (0.001 %, pH 7) 585 ± 5 nm

A 1 cm/λmax./1 % pH 7 ca. 500

Storage Temperature +15 °C TO +30 °C