BCA Protein Assay Macro Kit, Serva
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The assay bases on the bichinchoninic acid method (1). Proteins reduce alkaline Cu(II) to Cu(I). Bichinchoninic acid forms a purple complex with Cu(I) with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration.
Fast and sensitive assay: linear detection range from 25 – 1000 µg protein/ml
Easy to use: contains ready-to-use reagents and protein standard
Compatible with many detergents
Less binding variation between different proteins than Bradford assay
References:
Smith, P.K., et al. (1985) Anal. Biochem. 150, 76 - 85
HS: 38229000
Storage Temperature: +2 °C to +8 °C
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