5-Bromo-4-chloro-3-indolyl-phosphate-p-toluidine-salt, research grade, SERVA

Opis towaru

5-bromo-4-chloro-3-indolyl phosphate (BCIP) is a histochemical substrate for phosphatase. BCIP is used in conjunction with Nitro Blue Tetrazolium (NBT, cat. no. 30550) for the colorimetric detection of alkaline phosphatase-labelled molecules in Northern, Southern, and Western blotting, in situ hybridization, and immunohistochemistry. When incubated with alkaline phosphatase the BCIP/NBT substrate produces an insoluble, purple coloured NBT diformazan precipitate that is easily detected on membranes or tissue sections.
BCIP p-toluidine salt and NBT should be solved in dimethylformamide for preparation of a stock solution.

BCIP
C₈H₆NO₄BrClP·C₇H₉N or C₁₅H₁₅BrClN₂O₄P 

M_r 433.6

CAS No. 6578-06-9  

Assay (HPLC): min. 99.0 %

Storage Temperature -15 °C TO -25 °C