5-Bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal), research grade, SERVA

Opis towaru

X-Gal is a well known histochemical substrate used to detect the β-galactosidase enzyme. Identification of lac^f-colonies. X-gal is used to distinguish recombinant plasmids from parental vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene. Upon hydrolysis, X-Gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. X-Gal has been used for the detection of coliforms (E. coli) in municipal water supplies and food products. X-Gal is often used in conjunction with IPTG (Isopropyl-β-D-thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing β-galactosidase activity.
Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the dark. For detection of transformants, use in final concentration of 40 µg/ml. 

X-Gal
C₁₄H₁₅BrClNO₆ 

M_r 408.6 

CAS No. 7240-90-6

Purity (HPLC): min. 99.0 %

Storage Temperature: +2 °C to +8 °C