3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromide, research grade, SERVA

Opis towaru

Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.

Assay (HPLC) min. 98.0 %

WARNING

Hazard StatementsH315 - H319 - H335 - H341

PrecautionsP261 - P280

ReactionP302 +P352 - P304 +P340 - P305 +P351 +P338 - P337 +P313

CAS No. 298-93-1

(MTT, Thiazolyl blue)

C₁₈H₁₆BrN₅S

M_r 414.33

Storage Temperature: +15 °C to +30 °C