3,3'-Diaminobenzidine-4HCl-xH2O, research grade, SERVA

Opis towaru

3,3'-Diaminobenzidine tetrahydrochloride (DAB) is a substrate for peroxidases, especially for horseradish peroxidase (HRP).

DAB is used for histology and ultrahistochemistry (1) and demonstration of ultrastructural peroxidase (2).

It serves as a hydrogen donor in the presence of peroxide in the peroxidase reaction. The oxidized DAB forms an insoluble brown end-product, which can be detected in visible light and does not bleach during long-term storage.

Stock solution: 10 - 20 mg/ml in 50 mM Tris buffer, pH 7.3 (store in aliquots at -20 °C), working solution: 0.5 - 1 mg/ml in buffer (PBS, TBS, pH 7.0 - 7.6) containing 0.01 % hydrogen peroxide.

Vials under argon.

Assay (HPLC) min. 96.0 %

CAS No. 868272-85-9

(DAB, Diaminobenzidine tetrahydrochloride)

C₁₂H1₄N₄·4HCl·xH₂O

M_r 360.1 (anhydr.)

Storage Temperature: +2 °C to +8 °C